Antimetabolite Flucytosine (FC) and its specific mode of action was deeply investigated. FC appeared to have an unique mechanism of action, that is not shared with other antimetabolites. Other antimetabolites failed to inhibit Pseudomonas virulence, despite showing some antibiofilm activity. Since RNase E, an essential protein involved in RNA turnover, was shown to be the target of FC, researchers hypothize that other inhibitors of RNase E can be identified. They might have antivirulence effect similar to FC, but higher efficacy in vivo and lower risk of long term toxicity.