Researchers showed that treatment with ABL/PI5P and/or Metformin rescues impaired phagosome acidification in CFTR-pharmacologically inhibited macrophages and promotes intracellular bacterial killing in CF primary macrophages infected with Multiple-Drug-Resistance (MDR) P. aeruginosa strain, although only ABL/PI5P stimulation increase bacterial uptake. Finally, preliminary results in in vivo model of MDR-RP73 P. aeruginosa acute infection, show that treatment with ABL/PI5P or Metformin induces an overall decrease in leukocytes recruitment, associated to an increase of macrophage component and to a reduction of pulmonary bacterial load, although following ABL/PI5P treatment only. These results show that bioactive liposome and Metformin-based strategy could represent a promising host-directed therapeutic option for the control of drug-resistant bacterial infections and for the reduction of the inflammation-based pathology in CF.

Pubblicazioni

– Nisini R, Poerio N, Mariotti S et al. “The Multirole of Liposomes in Therapy and Prevention of Infectious Diseases” Frontiers in Immunology 2018 Feb 5;9:155