Developed skills and lines of research
Olga Luisa Angelica Zegarra (1955 – 2017) was Group leader of the Molecular Genetics laboratory at the Giannina Gaslini Institute in Genoa, in close collaboration with Luis Galietta. She obtained a degree in Medicine from the Universidad Central de Venezuela in 1983, and a second degree in Biology from the University of Genoa in 2008. An expert in physiology and cell biology, she was Principal Investigator, Partner and collaborator in many research projects on cystic fibrosis. The last line of research in cystic fibrosis developed by her concerned CF bronchial mucus and its alteration due to the bicarbonate secretion defect.
Projects funded by FFC Ricerca as Principal Investigator or as Research Manager
FFC#12/2016
Properties of airway mucus in cystic fibrosis: their modification by changes in the activity of CFTR and after application of bicarbonate
FFC#29/2014
Properties of airway mucus in cystic fibrosis: their modification by changes in the activity of CFTR and after application of bicarbonate
FFC#7/2009
Strategie per la soppressione dell’iperassorbimento di Na+ e fluido nella malattia delle vie aeree in fibrosi cistica
FFC#7/2003
Proteomica del liquido di superficie delle vie aeree: implicazioni per la fibrosi cistica
Projects financed by FFC Ricerca as a partner
FFC #2/2008
Basi funzionali e strutturali del meccanismo molecolare dei potenziatori della CFTR
FFC#4/2006
Basi funzionali e strutturali del meccanismo molecolare dei potenziatori della CFTR: verso molecole potenzialmente terapeutiche
FFC#2/2003
Attivatori del trasporto ionico: identificazione e modellazione molecolare dei siti leganti
Publications from FFC Research projects
Candiano G, Bruschi M, Pedemonte N, et al. Gelsolin secretion in interleukin-4-treated bronchial epithelia and in asthmatic airways. Am J Respir Crit Care Med, 2005 Nov 1;172(9):1090-6. Epub 2005 Aug 11.
Candiano G, Bruschi M, Pedemonte N, et al. Proteomic analysis of the airway surface liquid: modulation by proinflammatory cytokines. Am J Physiol Lung Cell Mol Physiol, 2007 Jan;292(1):L185-98.
Gianotti A, Melani R, Caci E, et al. Epithelial sodium channel silencing as a strategy to correct the airway surface fluid deficit in cystic fibrosis. Am J Respir Cell Mol Biol, 2013 Sep;49(3):445-52. doi: 10.1165/rcmb.2012-0408OC.
Stigliani M, Manniello MD, Zegarra-Moran O, et al. Rheological properties of cystic fibrosis bronchial secretion and in vitro drug permeation study: the effect of sodium bicarbonate. J Aerosol Med Pulm Drug Deliv, 2016 Aug;29(4):337-45. doi: 10.1089/jamp.2015.1228. Epub 2016 Jan 7.
Gianotti A, Melani R, Caci E, et al. ENaC Silencing as a Strategy to Correct the Airway Surface Fluid Deficit in Cystic Fibrosis. Am J Respir Cell Mol Biol, 2013 Sep;49(3):445-52. doi: 10.1165/rcmb.2012-0408OC.
Gianotti A, Capurro V, Scudieri P et al. Pharmacological rescue of mutant CFTR protein improves the viscoelastic properties of CF mucus. J Cyst Fibros. 2016 May;15(3):295-301. doi: 10.1016/j.jcf.2015.11.003. Epub 2015 Dec 9.
Auriche C, Di Domenico EG, Pierandrei S et al. CFTR expression and activity from the human CFTR locus in BAC vectors, with regulatory regions, isolated by a single-step procedure. Gene Ther. 2010 Nov;17(11):1341-54. doi: 10.1038/gt.2010.89. Epub 2010 Jun 10.