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Rampioni Giordano

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Rampioni Giordano

INSTITUTE

Università degli Studi Roma Tre

Email

giordano.rampioni@uniroma3.it

ADDRESS

Dipartimento di Scienze

PHONE

06 57336351

Developed skills and lines of research

Giordano Rampioni graduated in Biological Sciences in 2003 at Roma Tre University and earned a PhD in Biology Applied to Human Health in 2007 at the same institution. After several years of postdoctoral research in the United States at the University of Washington in Seattle and in England at the University of Nottingham, he became a researcher in 2011 at the Department of Sciences at Roma Tre University, where he has been an Associate Professor since 2022.

His research has focused on the study of gene regulation in bacteria of the Pseudomonas genus, with particular attention to the molecular mechanisms underlying intercellular communication processes. In Pseudomonas aeruginosa, these processes regulate the production of virulence factors and biofilm formation. In the context of cystic fibrosis, he contributed to developing bacterial biosensors that have been used in screening campaigns to reposition already-approved drugs for treating lung infections caused by P. aeruginosa.

He has authored over 60 publications in international journals, with more than 1,900 citations (Scopus), and holds a patent.

Projects funded by FFC Ricerca as Principal Investigator or as Research Manager

FFC#10/2023
Drug repurposing to inhibit Pseudomonas aeruginosa adaptation to the CF lung environment

FFC#17/2018
Drug repurposing for antivirulence therapy against Pseudomonas aeruginosa

FFC#13/2011
Identification and characterization of novel drugs suppressing Pseudomonas aeruginosa virulence in chronic infection

FFC#8/2008
Development and validation of a novel screening system for the identification of Pseudomonas aeruginosa virulence inhibitor

FFC#10/2007
Iron uptake and quorum sensing in Pseudomonas aeruginosa virulencee


Projects financed by FFC Ricerca as a partner

FFC#8/2022
Targeting the STING/Transglutaminase 2-regulated Interferon response as a novel host-direct approach to fight bacterial infections in cystic fibrosis

FFC#15/2020
Targeting the STING/Transglutaminase 2-regulated Interferon response as a novel host-direct approach to fight bacterial infections in Cystic Fibrosis

FFC#4/2019
Restoring defective proteostasis in Cystic Fibrosis: novel strategies for F508del-CFTR repair

FFC#27/2015
Intra-individual biological variation in sweat chloride concentrations

FFC#20/2012
Early antibiotic treatment for MRSA eradication in cystic fibrosis patients: a randomised multicentre study

Publications from FFC Research projects

  • Rampioni G, Schuster M, Greenberg EP, Bertani I, Grasso M, Venturi V, Zennaro E, Leoni L (2007) RsaL provides quorum sensing homeostasis and functions as a global regulator of gene expression in Pseudomonas aeruginosa. Mol Microbiol 66:1557-1565. doi:10.1111/j.1365-2958.2007.06029.x.
  • Rampioni G, Schuster M, Greenberg EP, Zennaro E, Leoni L (2009) Contribution of the RsaL global regulator to Pseudomonas aeruginosa virulence and biofilm formation. FEMS Microbiol Lett 301:210-217. doi:10.1111/j.1574-6968.2009.01817.x.
  • Venturi V, Rampioni G, Pongor S, Leoni L (2011) The virtue of temperance: built-in negative regulators of quorum sensing in Pseudomonas. Mol Microbiol 82:1060-1070. doi:10.1111/j.1365-2958.2011.07890.x.
  • Imperi F, Massai F, Ramachandran Pillai C, Longo F, Zennaro E, Rampioni G, Visca P, Leoni L (2013) New life for an old drug: the anthelmintic drug niclosamide inhibits Pseudomonas aeruginosa quorum sensing. Antimicrob Agents Chemother 57:996-1005. doi:10.1128/AAC.01952-12.
  • Longo F, Rampioni G, Bondì R, Imperi F, Fimia GM, Visca P, Zennaro E, Leoni L (2013) A new transcriptional repressor of the Pseudomonas aeruginosa quorum sensing receptor gene lasR. PLoS One 8:e69554. doi:10.1371/journal.pone.0069554.
  • Rampioni G, Leoni L, Williams P (2014) The art of antibacterial warfare: Deception through interference with quorum sensing-mediated communication. Bioorg Chem 55:60-68. doi:10.1016/j.bioorg.2014.04.005.
  • Bondí R, Messina M, De Fino I, Bragonzi A, Rampioni G, Leoni L (2014) Affecting Pseudomonas aeruginosa phenotypic plasticity by quorum sensing dysregulation hampers pathogenicity in murine chronic lung infection. PLoS One 9:e112105. doi:10.1371/journal.pone.0112105.
  • Costabile G, d’Angelo I, Rampioni G, Bondì R, Pompili B, Ascenzioni F, Mitidieri E, d’Emmanuele di Villa Bianca R, Sorrentino R, Miro A, Quaglia F, Imperi F, Leoni L, Ungaro F (2015) Toward repositioning niclosamide for antivirulence therapy of Pseudomonas aeruginosa lung infections: Development of inhalable formulations through nanosuspension technology. Mol Pharm 12:2604-2617. doi:10.1021/acs.molpharmaceut.5b00098.
  • Fernicola S, Torquati I, Paiardini A, Giardina G, Rampioni G, Messina M, Leoni L, Del Bello F, Petrelli R, Rinaldo S, Cappellacci L, Cutruzzolà F (2015) Synthesis of triazole-linked analogues of c-di-GMP and their interactions with diguanylate cyclase. J Med Chem 58:8269-8284. doi:10.1021/acs.jmedchem.5b01184.
  • Fernicola S, Paiardini A, Giardina G, Rampioni G, Leoni L, Cutruzzolà F, Rinaldo S (2015) In silico discovery and in vitro validation of catechol-containing sulfonohydrazide compounds as potent inhibitors of the diguanylate cyclase PleD. J Bacteriol 198:147-156. doi:10.1128/JB.00742-15.
  • Pawar SV, Messina M, Rinaldo S, Cutruzzolà F, Kaever V, Rampioni G, Leoni L (2016) Novel genetic tools to tackle c-di-GMP-dependent signalling in Pseudomonas aeruginosa. J Appl Microbiol 120:205-217. doi:10.1111/jam.12984.
  • Rampioni G, Falcone M, Heeb S, Frangipani E, Fletcher MP, Dubern JF, Visca P, Leoni L, Cámara M, Williams P (2016) Unravelling the genome-wide contributions of specific 2-alkyl-4-quinolones and PqsE to quorum sensing in Pseudomonas aeruginosa. PLoS Pathog 12:e1006029. doi:10.1371/journal.ppat.1006029.
  • Rampioni G, Visca P, Leoni L, Imperi F (2017) Drug repurposing for antivirulence therapy against opportunistic bacterial pathogens. Emerg Top Life Sci 1:13-22. doi:10.1042/ETLS20160018.
  • Bondí R, Longo F, Messina M, D’Angelo F, Visca P, Leoni L, Rampioni G (2017) The multi-output incoherent feedforward loop constituted by the transcriptional regulators LasR and RsaL confers robustness to a subset of quorum sensing genes in Pseudomonas aeruginosa. Mol Biosyst 13:1080-1089. doi:10.1039/c7mb00040e.
  • Rampioni G, Pillai CR, Longo F, Bondì R, Baldelli V, Messina M, Imperi F, Visca P, Leoni L (2017) Effect of efflux pump inhibition on Pseudomonas aeruginosa transcriptome and virulence. Sci Rep 7:11392. doi:10.1038/s41598-017-11892-9.
  • D’Angelo F, Baldelli V, Halliday N, Pantalone P, Polticelli F, Fiscarelli E, Williams P, Visca P, Leoni L, Rampioni G (2018) Identification of FDA-approved drugs as antivirulence agents targeting the pqs quorum-sensing system of Pseudomonas aeruginosa. Antimicrob Agents Chemother 62:e01296-18. doi:10.1128/AAC.01296-18.
  • Mellini M, Di Muzio E, D’Angelo F, Baldelli V, Ferrillo S, Visca P, Leoni L, Polticelli F, Rampioni G (2019) In silico selection and experimental validation of FDA-approved drugs as anti-quorum sensing agents. Front Microbiol 10:2355. doi:10.3389/fmicb.2019.02355.
  • Baldelli V, D’Angelo F, Pavoncello V, Fiscarelli EV, Visca P, Rampioni G, Leoni L (2020) Identification of FDA-approved antivirulence drugs targeting the Pseudomonas aeruginosa quorum sensing effector protein PqsE. Virulence 11:652-668. doi:10.1080/21505594.2020.1770508.
  • Mellini M, Lucidi M, Imperi F, Visca P, Leoni L, Rampioni G (2021) Generation of genetic tools for gauging multiple-gene expression at the single-cell level. Appl Environ Microbiol 87:e02956-20. doi:10.1128/AEM.02956-20.
  • Fortuna A, Bähre H, Visca P, Rampioni G, Leoni L (2021) The two Pseudomonas aeruginosa DksA stringent response proteins are largely interchangeable at the whole transcriptome level and in the control of virulence-related traits. Environ Microbiol 23:5487-5504. doi:10.1111/1462-2920.15693.
  • Visaggio D, Frangipani E, Hijazi S, Pirolo M, Leoni L, Rampioni G, Imperi F, Bernstein L, Sorrentino R, Ungaro F, Visca P (2022) Variable susceptibility to gallium compounds of major cystic fibrosis pathogens. ACS Infect Dis 8:78-85. doi:10.1021/acsinfecdis.1c00409.
  • Collalto D, Giallonardi G, Fortuna A, Meneghini C, Fiscarelli E, Visca P, Imperi F, Rampioni G, Leoni L (2022) In vitro activity of antivirulence drugs targeting the las or pqs quorum sensing against cystic fibrosis Pseudomonas aeruginosa isolates. Front Microbiol 13:845231. doi:10.3389/fmicb.2022.845231.
  • Letizia M, Mellini M, Fortuna A, Visca P, Imperi F, Leoni L, Rampioni G (2022) PqsE expands and differentially modulates the RhlR ruorum sensing regulon in Pseudomonas aeruginosa. Microbiol Spectr 10:e0096122. doi:10.1128/spectrum.00961-22.