Tissue engineering science aims to recreate tissues and organs in order to restore their original biological functions; and more to build up pathological tissues/organs to provide in vitro platforms, in replacement of the in vivo model, on which studying CF disease and testing new therapeutic approaches. In this project, researchers want to engineer a full thickness airway tissue in-vitro model of CF disease (FT_CF). To this aim, they will build up a lung connective tissue where bronchial epithelial cells from CF patients will be induced to differentiate in a thickness stratified mucociliated epithelium. The insertion, also, of the engineered CF model in a microfluidic chip, makes possible the tissue culture in controlled fluid-dynamic conditions and appropriately modulated to promote epithelial differentiation. The final object is a novel in vitro CF model to predict and monitor the potential efficacy of new therapies finalized to correct the CFTR defect.