In recent years some correctors able to rescue F508del-CFTR were identified, but their mechanism and binding sites were not clarified. So this project aims to unveil the F508del-CFTR domains involved in binding capacity and the differences among F508del correctors and wild type CFTR binding. The additive and synergistic effects of combination of correctors binding to different CFTR regions will be studied. Complementary biochemical experiments will be carried out on samples of mammalian cells transfected either with wild type CFTR and F508del-CFTR whole molecules but also with single or multiple protein domains.