Epigenetic regulation of pro-inflammatory genes and direct regulation of CFTR protein expression by MicroRNA has been reported. The objectives of the project MicroRNA-CF are: identification of specific MicroRNAs and their most relevant mRNA targets in CF bronchial epithelial cells; study of changes of the gene expression and secretome profile of CF using PNAs targeting MicroRNAs; development and biological validation of novelPNA-based molecules interfering with MicroRNAs involved in inflammation; development of PNA-based antagomiRNAs for CFTR stabilization.