Cystic Fibrosis (CF) is a complex disease which includes impaired chloride permeability and persistent inflammation and might benefit from multifactorial therapeutic approaches. The aim of this proposal is centered around the effects in CF of thymosin alpha1, a naturally occurring polypeptide (ZADAXIN®) used in different pathologies mainly to modulate immunity and inflammation. Since rescuing of the chloride channel activity may ameliorate the inflammatory picture in CF chronic disease, the researchers hypothesize that the reverse is possible, that is regulation of inflammation may improve CFTR functioning. They carry out the study investigating in pancreatic and gut cells and CF mice models (homozygous for the F508del-CFTR) both the aspects: on one side the thymosin alpha1 the ability to regulate autophagy, inflammation and antimicrobial resistance, on the other to modulate CFTR activity and to rescue its chloride channel function. The final aim is to provide data for repurposing a drug approved for other indications for the treatment of CF.