Mycobacterium abscessus (MA) is an emerging multidrug resistance (MDR) non-tuberculous mycobacterium (NTM) that affects cystic fibrosis (CF) patients, and is often associated with an important decline in lung function. Liposomes carrying bioactive lipids represent a new pharmacologic approach that is able to enhance bactericidal innate immunity against MDR pulmonary infections. The main goal of the present study, that is an extension of FFC#16/2018, will be to evaluate the synergistic effect of different liposomes with and without an antibiotic treatment on in vitro and in vivo MA infection. The synergistic effect of different liposomes with and without the antibiotic therapy will be validated in vitro, in wild type and CFTR-/- mice chronically infected with MA. Magnetic resonance imaging (MRI) will be applied in longitudinal measurements to monitor changes in infectious lesions within the same animal. Liposomes delivering bioactive lipid could represent a novel immunotherapeutic strategy to treat pulmonary infection by drug-resistant MA in CF patients.