The therapy based on phages, virus which are natural enemies of the bacteria, is garnering renewed interest as bacterial resistance to antibiotics is becoming increasingly widespread. Advantages on antibiotic therapy are that bacteriophages: 1) multiply at the infection site, increasing their number, whereas antibiotics are metabolized and eliminated from the body; 2) target only specific bacteria, with no effect on commensal flora; 3) can overcome bacterial resistance; 4) have the capacity to reach bacteria trapped inside biofilms. Aim of this project was the characterization of several phages used currently in Eastern Europe, testing their ability to infect Italian P. aeruginosa clinical strains, and to isolate novel phages from Italian samples. Moreover, the ability of the phages to dissolve the biofilms formed by P. aeruginosa clinical strains was evaluated.