The main objective of the present project was to discover novel compounds able to interfere with the resistance mechanism of bacteria such as P. aeruginosa. Metallo-beta-lactamase are enzymes responsible for bacterial resistance. The research plan was to discover inhibitors of MBL and through these inhibitors prevent the resistance. Design of inhibitors was performed through computer-aided methodologies, the compounds were then synthesized and tested against the enzymes and finally on bacterial cultures. The expected output of the project was the selection of the above mentioned inhibitors. The perspective was the development of innovative antibacterial therapies, based on the combination of antibiotics and beta-lactamase inhibitors, useful for the treatment of patients with cystic fibrosis who do not respond to the usual antibiotic treatment.