In their previous project FFC#8/2017 researchers, experts in the field of tissue-engineering, developed a novel full-thickness cystic fibrosis (CF) model on a microfluidic chip. The main innovative feature of this model is the presence of both the pulmonary epithelial and connective compartments. Currently, airways in vitro models are not provided by connective compartment, so the role of epithelial/stromal crosstalk during CF remained little known. Since research data indicate existing epithelial-stromal crosstalk, in this project it will be investigated. Moreover, each component of the whole model (epithelium, stroma, and glands) will be characterized by a morphological and transcriptomics point of view. The model will be also implemented with an aerosol system for drug delivery and analysis of the airways surface liquid (ASL). In particular, to mimic infection and trigger inflammation, bacterial supernatants to the epithelial surface will be delivered, as well as CF modulator therapy (VX-809 / VX-770). In this way, researchers will assess how they modify the properties of the apical fluid as well as the status of epithelial and stromal components. There is growing need and interest in developing in vitro human cystic fibrosis model and this novel full-thickness CF model on a microfluidic chip will help to better understanding pathogenic mechanism and evaluate novel therapeutic approach.