The main objective of the project is to determine the response of persons with cystic fibrosis (CF) from various CF centers to different modulators through the use of nasal cell cultures. This project is the extension of the previous FFC#9/2019 during which a network of Italian CF centers was created and persons with CF were characterized in terms of sensitivity to CFTR modulators. By means of nasal brushing, a minimally invasive procedure for obtaining nasal epithelial cells from persons with CF, nasal cells will be cultured and differentiated in vitro using specific protocols. On them, the research group will test the effect of modulators using electrophysiological techniques. The researchers also intend to evaluate possible secondary effects capable of altering the expression of CFTR due to mutations. The study will help in the selection of more effective drugs and define personalized treatments for each person with CF, including those with rare and not yet characterized mutations.

XIX Convention FFC Ricerca – download here a brief presentation of the project